The OXIFERM II TUBE SYSTEM: (inco etc see Enterotube II)

The OxiFerm II Tube System is a commercial product available from Becton-Dickinson and Company for identification of enteric Gram-negative OXIDASE positive intestinal bacilli. The Enterotube II (see button below) is utilized for Gram-negative OXIDASE negative rods.  The oxiferm has the following test chambers: anaerobic glucose, arginine, lysine, lactose/nitrogen gas, sucrose/saccharose/indole, xylose, aerobic glucose, maltose, mannitol, phenylalanine, urea, and citrate.

Under the RED colored cap of the OxiFerm II tube you will find a wire handle.  The sterile wire inserted in the oxiferm tube is used to obtain growth from an isolated colony of the test organism. The wire is pushed down through the twelve compartments in a twisting motion (3X do not remove wire between times) inoculating each test as it proceeds.  Break the wire at the "score" on the wire on the third insertion and use it to punch a hole in the compartments indicated on the insert at the "windows."  Re-cap the tube.  If your OxiFerm II tube has a removable paper cover for holes for these chambers you do not need to punch them with the wire... just, pull the paper strip off the tube after inoculation.  Label the tube with the date and incubate "flat side-down." 

Results are recorded after 18-24 hours of incubation using a specific oxiferm form and reference Identicode Guidebook. Remember to record the chamber colors immediately after incubation and BEFORE adding the reagent for the indole test as the reagent changes the chamber colors.  Look for bubbles of gas trapped against the bottom of the first four chambers. 

Kovacs Reagent should be added to that chamber by piercing the plastic wrapping with a pen and adding one drop to the chamber - observe for a pink color change...

Discard of the wire and the entire OxiFerm II tube in a suitable BIOHAZARD container when you are finished reading it...

Click on the BLUE button below to follow that link...